- 专利标题: System for measuring anomalous diffusion using fluorescence recovery after photobleaching and associated method
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申请号: US17083365申请日: 2020-10-29
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公开(公告)号: US11585755B2公开(公告)日: 2023-02-21
- 发明人: Minchul Kang
- 申请人: THE TEXAS A&M UNIVERSITY SYSTEM
- 申请人地址: US TX College Station
- 专利权人: THE TEXAS A&M UNIVERSITY SYSTEM
- 当前专利权人: THE TEXAS A&M UNIVERSITY SYSTEM
- 当前专利权人地址: US TX College Station
- 代理机构: Allen, Dyer, Doppelt + Gilchrist, P.A.
- 主分类号: G01N21/64
- IPC分类号: G01N21/64 ; G02B21/00
摘要:
A system and associated method measures anomalous diffusion of biomolecules in cell membranes of intact cells and includes a laser that illuminates a cell membrane within an intact cell to express fluorescently tagged biomolecules. The laser photobleaches a region of interest and illuminates the region of interest over time. A detector detects the fluorescence recovery over time within the region of interest to yield fluorescence recovery after photobleaching (FRAP) data. A controller computes the mean square displacement (MSD) of diffusing biomolecules and a time-dependent diffusion coefficient D(t) from a plurality of time points of the FRAP data and determines the anomalous diffusion in the cell membrane.