A signal receiver includes a data sampler receiving a differential input signal having first and second input signals and determining bit values of the differential input signal based on first and second reference voltages, and a reference voltage generator performing a pre-tuning operation and a post-tuning operation to generate the reference voltages. The reference voltage generator performs the pre-tuning operation by generating first and second initial voltages and adjusting one of the initial voltages to generate third and fourth voltages. After the pre-tuning operation, the reference voltage generator performs the post-tuning operation by increasing or decreasing the third voltage to generate the first reference voltage and decreasing or increasing the fourth voltage to generate the second reference voltage based on a comparison result between the third voltage and the first input signal and a second comparison result between the fourth voltage and second input signal.