A network communication power supply with digital signal isolation includes a transformer, a transformer drive circuit, a rectifier, and a modulation signal duty cycle detection circuit. The transformer has a primary side and a secondary side. The transformer drive circuit is coupled to the primary side, and receives a digital signal input. The transformer drive circuit converts the digital signal input into a drive signal with a duty cycle corresponding to a logic level of the digital signal input according to the logic level. The digital signal input includes a power content. The rectifier is coupled to the secondary side and converts the power content to provide a power source. The modulation signal duty cycle detection circuit is coupled to the rectifier and supplied power by the power source, and provides a digital signal output with a high or low level according to the duty cycle.