Provided are a transfer substrate, a display panel and a transfer method. The transfer substrate includes a plurality of object setting regions arranged in an array, the plurality of object setting regions including n types, where n is a positive integer, and n≥2. The transfer substrate further includes: a base substrate, and a blocking layer located on a side of the base substrate. The blocking layer forms accommodating grooves respectively within object setting regions. Phase change materials are provided in accommodating grooves of at least (n−1) types of object setting regions. The provided transfer substrate has a simple structure and high transfer efficiency.