The present application provides a display substrate, the display substrate including a plurality of sub-pixel units, the plurality of sub-pixel units arranged in a plurality of rows and columns, wherein the plurality of columns of the sub-pixel units include alternatingly disposed solid color sub-pixel unit columns and mixed color sub-pixel unit columns, the solid color sub-pixel unit columns including a plurality of sub-pixel units corresponding to the same color, and in the mixed color sub-pixel unit columns, adjacent two sub-pixel units correspond to different colors. In the same row of sub-pixel units, the sub-pixel units on both sides of the sub-pixel unit in the solid color sub-pixel unit column have different colors. The utility model also provides a display device. The display device does not appear jagged or blurred when displaying an image.