A display substrate and a display device are disclosed. The display substrate includes a base substrate, an insulating layer, a first crack stopper, and a first crack detection line. The base substrate includes a display region and a non-display region. The insulating layer is located on the base substrate. The first crack stopper is located in the non-display region and is configured to block the first crack in the insulating layer from extending towards the display region. The first crack detection line is located in the non-display region, an edge of the orthographic projection of the first crack stopper on the base substrate close to the display region is a blocking edge, and the orthographic projection of the first crack detection line on the base substrate is located at a side of the orthographic projection of the first crack stopper on the base substrate away from the blocking edge.