- 专利标题: Linker composition comprising silica-forming peptides
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申请号: US16793081申请日: 2020-02-18
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公开(公告)号: US11633491B2公开(公告)日: 2023-04-25
- 发明人: Seung Pil Pack , Mohamed Abdeltawab Abdallah Abdelhamid , Ki Baek Yeo , Mi Ran Ki
- 申请人: Korea University Research and Business Foundation, Sejong Campus
- 申请人地址: KR Sejong
- 专利权人: Korea University Research and Business Foundation, Sejong Campus
- 当前专利权人: Korea University Research and Business Foundation, Sejong Campus
- 当前专利权人地址: KR Sejong
- 代理机构: NSIP Law
- 优先权: KR10-2019-0017807 20190215,KR10-2020-0013232 20200204
- 主分类号: A61K47/65
- IPC分类号: A61K47/65 ; A61K47/64
摘要:
The present disclosure relates to the use of silica-forming peptides (SFPs) Volp1 and Salp1 for protein fusion tags for protein purification, silica formation and self-encapsulation, and controlled release of biomolecules. After preparing a fusion protein containing the Volp1 or Salp1 peptide at the C-terminal of the protein, the adsorption conditions for silica (SiO2) surface were optimized. As a result, a high-purity fusion protein could be purified without an additional tag for purification. The fusion protein containing the silica-forming peptide was self-encapsulated and stably immobilized in the silica matrix through reaction with a silica precursor. The Volp1 and Salp1 peptides showed stronger adsorption ability for silica than the previously known R5 peptide. Using these characteristics, a silica complex wherein two proteins are encapsulated was prepared by mixing the R5 fusion protein with the Volp1 or Salp1 fusion protein. The resulting controlled release system allows the release of the R5 fusion protein in the first step and release of the Salp1 fusion protein in the second step based on the difference in adsorption affinity.
公开/授权文献
- US20200261593A1 LINKER COMPOSITION COMPRISING SILICA-FORMING PEPTIDES 公开/授权日:2020-08-20