The light extraction device, the detection equipment and the operation method thereof are provided. The light extraction device includes at least one light splitting unit, each of the at least one light splitting unit includes a color separation grating, configured to separate light incident on the color separation grating into a plurality of light beams that are collimated and propagated in different directions and have different colors; a first lens, disposed corresponding to the color separation grating and configured to converge the plurality of light beams; and a first pinhole, located on a side of the first lens away from the color separation grating and correspondingly arranged with the first lens. The first lens is configured to converge a light beam having a preset color in the plurality of light beams to the first pinhole and allow the light beam having the preset color to exit.