A strict reverse navigation method for optimal estimation of fine alignment is provided. The strict reverse navigation method including: establishing an adaptive control function; performing a forward navigation calculation process; performing a reverse navigation calculation process; and performing the adaptive control for a number of forward and reverse calculations. The strict reverse navigation method shortens an alignment time for the optimal estimation of fine alignment while ensuring an alignment accuracy. The strict reverse navigation method provided effectively solves a problem that an error of an initial value of filtering in an initial stage of the optimal estimation of fine alignment affects convergence speeds of subsequent stages. In the initial stage, a larger number of the forward and reverse navigation calculations are adopted to reduce an error of the initial value as much as possible and increase a convergence speed of the filtering.