A touch display substrate and a display device are provided. The touch display substrate includes a plurality of sub-pixel units (10), data lines (20) and touch signal lines (30). For any two adjacent rows of sub-pixel units, the sub-pixel unit (10) in one row of sub-pixel units is staggered in a row direction with respect to the sub-pixel unit (10) in the other row of sub-pixel units adjacent to the one row of sub-pixel units by a distance of X sub-pixel units (10), and 0