Methods, apparatus, systems, and articles of manufacture to create a reference signature asset from matched signature fragments are disclosed. Example apparatus disclosed herein form a group of matched signature fragments corresponding to a same piece of media. Disclosed example apparatus also select a subset of matched signature fragments from the group to form a path of matched signature fragments that collectively represent a continuous segment of the piece of media, the continuous segment identified by a segment start time and a segment end time, the segment start time corresponding to a fragment start time associated with an initial matched signature fragment in the path, and the segment end time corresponding to a fragment end time associated with a last matched signature fragment in the path. Disclosed example apparatus further use the path to combine the subset of matched signature fragments and create a new reference signature asset.