A toolholder matched with the internal jet cooling spindle for cryogenic coolant is mainly composed of a hollow toolholder body, a high-performance thermal insulation structure and a bidirectional sealing structure. They can guide the cryogenic coolant from the spindle to the internal cooling channel of cutting tool and realize the cryogenic thermal insulation and dynamic sealing. The high-performance thermal insulation structure inside the toolholder employs the material with a low thermal conductivity and a low linear expansion coefficient to restrain the low temperature impact of cryogenic coolant on the toolholder and spindle, to ensure the dimensional accuracy and assembly accuracy of the toolholder. The bidirectional sealing structure in the toolholder uses the ultra-low temperature resistant seal rings to prevent the cryogenic coolant from leaking towards the spindle and the cutting tool, to ensure the stability of the coolant transport.