A mini-LED backlight structure and a backlight module are provided. The mini-LED backlight structure includes a backplate, a glass substrate, and reflective sheets. The glass substrate includes a line surface and a lineless surface close to a side of the backplate. Light emitting diodes and a connector are disposed on the lineless surface. Furthermore, a gap is between the backplate and the glass substrate for disposing the light emitting diodes and the connector. The connector is electrically connected to an external circuit through a conducting wire. Disposing a plurality of elements, the connector, and the light emitting diodes on a same surface of the lineless surface is unlikely affecting backlight effect and saves materials at a same time.