A zero-Poisson-ratio honeycomb structure and an interlocking assembly manufacturing method thereof are provided. The honeycomb structure is formed by combining a four-pointed star shaped structure and horizontal and vertical honeycomb wall arrays at star corners. The zero-Poisson-ratio honeycomb structure not only has the zero-Poisson-ratio characteristic, but also can achieve respective design of in-plane and out-of-plane mechanical properties. Meanwhile, due to the existence of the horizontal honeycomb walls and the vertical honeycomb walls, the connection of multiple honeycomb walls at angular points in the honeycomb structure is avoided. Moreover, a novel manufacturing mode is provided for the honeycomb structure in addition to prepare the honeycomb structure by utilizing a 3D printing process. The honeycomb structure can be manufactured by combining an interlocking assembly process with resin matrix composites. The performance of the honeycomb structure is further improved at the material level.