The present invention relates to an active noise cancellation integrated circuit for stacking multiple anti-noise signals, an associated method, and an active noise cancellation headphone using the same. The method is applicable to an audio playback device with multiple ANC filtering units. The method includes: acquiring an anti-noise signal from an ANC filtering unit; generating a decoupled signal by processing the anti-noise signal with the transfer function of a physical channel and operations of other ANC filtering units; performing a signal superposition, wherein an anti-noise signal from another ANC filtering unit is superposed with the decoupled signal; and performing an audio playback based on the superposed signal and an audio signal such that noise is eliminated.