A display substrate and a display apparatus are provided, the display substrate includes: a base substrate including a display area and a peripheral area surrounding the display area; a plurality of sub-pixel units in the display area; a plurality of data lines in the display area and electrically coupled to the plurality of sub-pixel units; a plurality of data transmission lines in the peripheral area on at least one side of the display area and electrically coupled to the plurality of data lines; a plurality of first pads and a plurality of second pads located between the plurality of first pads and the plurality of data transmission lines; a plurality of third pads between the plurality of first pads and the plurality of second pads; and a plurality of multiplexers between the plurality of second pads and the plurality of third pads.