A system and method for myocardial performance determination is provided. The present invention provided for generating a first dataset representing a set of events associated with a pre-defined parameter of a biomarker extracted from physiological parameters of a subject. The set of events is determined by processing the pre-defined parameter at a first level and a second level of a multi-level artificial neural network architecture recursively for a pre-defined number of times. Further, generating second dataset representing characteristics associated with the set of events by processing first dataset at third level and fourth level of multi-level artificial neural network architecture. Further, computing set of values associated with set of events by processing second dataset at fifth level of multi-level artificial neural network architecture. Further, computing myocardial performance index based on set of values. The myocardial performance index is representative of myocardial performance of the subject.