The present disclosure relates to a testing method and a testing system for a human stress reaction, and a computer-readable storage medium. The testing method for the human stress reaction includes acquiring position information and visual field information of a testee in a virtual road traffic scene after the virtual road traffic scene is established; guiding the testee into a test zone when the testee is within a test-waiting zone and when a visual field direction of the testee faces the test zone, and simultaneously, starting acquiring stress reaction data of the testee; controlling a virtual reality environment module to create a virtual stress event in the test zone after it is determined that the testee is within the test zone, and applying a stimulation to the testee, such that the testee make a stress reaction.