A portable and inflatable patient isolation chamber and stretcher system having an exoskeletal frame, which when inflated, forms a substantially tent contour enclosure when coupled to an inflatable flexible base. The exoskeletal frame has an apex section formed by a flexible apex conduit which is coupled to and in fluid communication with first and second flexible end conduits. The inflatable flexible base is connected to the exoskeletal frame and is in fluid communication with the exoskeletal frame for providing a flexible platform for the patient. A transparent envelope is secured to the exoskeletal frame and inflatable flexible base for forming a patient isolation chamber. When deflated, the flexible base can serve as a stretcher for transport of the patient.