A pixel structure driving method and a display device are provided. The driving method includes obtaining a polarity of each of the data lines of a current frame, determining a polarity of each of the sub-pixels according to the polarity of each of the data lines and an arrangement of the pixel structure, performing a value look-up process according to a polarity and an initial gray value of each of the sub-pixels to obtain a polarity high/low gray value of each of the sub-pixels, if a luminance of a high gray value of a positive polarity of a sub-pixel of a color is different from a luminance of a high gray value of a negative polarity of the sub-pixel of the color, adjusting the polarity high/low gray value of the sub-pixel. In this way, the occurrence of vertical strips in a still image could be alleviated.