A method for multi-sensor calibration includes imaging a calibration target with a first sensor using a first modality to obtain a first set of data and a second sensor using a second modality that is different from the first modality to obtain a second set of data. A border of the calibration target is identified based on the first set of data. A first centroid location of the calibration target is identified based on the border of the calibration target. A border of a pattern disposed on the calibration target is identified based on the second set of data. A second centroid location of the calibration target is identified based on the border of the pattern. Calibration data for the first sensor and the second sensor is generated based on the first centroid location and the second centroid location.