A video transmitting circuit and a signal delay compensation method thereof are provided. The video transmitting circuit transmits a specific signal through a first transmission path and a second transmission path to a video receiver during a calibration mode. In the calibration mode, a return detection circuit of the video transmitting circuit detects whether or not a first return signal transmitted through the first transmission path and a second return signal transmitted through the second transmission path have been received by the video transmitting circuit. The video transmitting circuit sets delay circuits serially connected in the first or second transmission path according to a detection result of the return detection circuit, such that the first return signal transmitted through the first transmission path and the second return signal transmitted through the second transmission path can synchronously arrive at the video receiver.