A pixel structure includes: a pixel definition layer having a first side and a second side which are opposite to each other in a thickness direction of the pixel definition layer, the pixel definition layer including a pixel opening having sidewalls; an island-shaped portion disposed in the pixel opening of the pixel definition layer and spaced apart from the sidewalls of the pixel opening, the island-shaped portion having sidewalls, and the sidewalls of the pixel opening and the sidewalls of the island-shaped portion defining a ring-shaped pixel groove; and a light emitting unit, at least a part of which is disposed in the ring-shaped pixel groove.