A display substrate and a display device, the display substrate includes a first side for displaying and a second side, and includes: a base substrate; a display region including first and second display regions, the first display region including multiple pixel unit groups, each pixel unit group including multiple first pixel units, and each first pixel unit including a pixel region and an opening region; multiple first power lines located in the pixel region; a shielding layer including a hollow region and a shielding region; for one pixel unit group, the opening region of each first pixel unit at least partially overlaps with the shielding region, the opening region of at least one first pixel unit includes a first shielding connecting portion at least partially overlapping with the shielding region, the shielding layer is connected with at least one first power line through the first shielding connecting portion.