A pump shaft support structure includes: a crankcase body which rotatably supports a crankshaft of an engine; a pump shaft which interlockingly rotates with the crankshaft; a crankcase cover which covers the crankcase body; a pump cover which is coupled to the crankcase cover to define a pump chamber therebetween; and a pump rotor which is disposed in the pump chamber and engages with the pump shaft. The pump shaft includes: a supported section which is rotatably supported by the pump cover on the other side of the pump chamber from the crankcase cover; and an insertion section adjoining the supported section in an axial direction. The pump cover has a through bore which passes the insertion section of the pump shaft therethrough. The insertion section of the pump shaft has a diameter smaller than that of the supported section.