Provided are a multipath separation method and device, and a storage medium. The multipath separation method includes: extracting frequency domain response characteristics of received reference signals in at least two different frequency bands; for each of the at least two different frequency bands, constructing a Toeplitz matrix; combining Toeplitz matrixes corresponding to the at least two different frequency bands; performing singular value decomposition on the synthesized Toeplitz matrix; determining a signal space matrix and a noise space matrix according to the decomposed matrix; constructing a plurality of frequency domain response vectors according to frequency domain response characteristics of local signals having different delays and are the same as the received reference signals; and comparing a first preset threshold with inner products between each of the plurality of frequency domain response vectors and the noise space matrix respectively, and determining a delay corresponding to each of a plurality of frequency domain response vectors in which inner products satisfy the first preset threshold to be a delay of one path in the multipath.