The present invention relates to a system and method for pupil tracking and eye-markers extracting using an image acquisition device such as a visible-light camera shooting a non-static head. In an embodiment of the present invention eye-markers are extracted from one eye or from both eyes. The extraction from both eyes uses for averaging the results of the two eyes when abnormalities are detected in one of the eyes. In addition, the invention relates to a computerized application, which interacts with a user (for example trough a game or movie, usually in a context of biofeedback), and takes video shooting of the users face and eyes during said interaction.