A refrigerator appliance or ice making assembly, as provided herein, may include a body and an ejector. The body may include an ice mold for receiving and freezing water. The ice mold may define a discrete first compartment and second compartment within which water freezes. The first compartment may define a first cube profile. The second compartment may be axially-spaced apart from the first compartment and define a second cube profile. The second cube profile may be different from the first cube profile. The ejector may be rotatably disposed above the first cube profile and the second cube profile to motivate ice from the first and second compartments.