A manufacturing method of an image pickup apparatus includes: fabricating a plurality of image pickup members each having a light-receiving surface on which a transparent plate is disposed and a plurality of spacers; measuring thicknesses of the transparent plates and the spacers; classifying the image pickup members into first groups depending on the thicknesses of the transparent plates; classifying the spacers into second groups depending on the thicknesses, selecting a combination of any one of the first groups and any one of the second groups such that a sum of the thickness of each of the transparent plates and the thickness of each of the spacers is within a predetermined range with a focusing length of optical members as a center; fabricating stacked bodies by stacking the image pickup members and the spacers in the selected combination; and disposing the optical members on the spacers in the stacked bodies.