The present disclosure relates to a display substrate, a display device and a manufacturing method of the display substrate. The display substrate includes: a stretchable base including a plurality of opening patterns distributed along a surface of the stretchable base, wherein each of the plurality of opening patterns includes a plurality of opening areas, a plurality of bridge areas configured to enclose a first island area are formed between adjacent opening areas among the plurality of opening areas, and each of the plurality of opening patterns is configured to enclose a plurality of second island areas with at least two adjacent opening patterns; a plurality of display units respectively arranged on the first island area and the plurality of second island areas; and a plurality of signal lines respectively connected between the plurality of display units and respectively arranged in the plurality of bridge areas.