Disclosed are a banknote stacking and separation apparatus and a cash recycling and handling device. The banknote stacking and separation apparatus includes a frame provided with a banknote accommodating space. A pickuping roller, a feeding roller and a gate roller are provided in an upper part of the banknote accommodating space, and the feeding roller and the gate roller are oppositely arranged. The banknote stacking and separation apparatus further includes a guiding plate and a guiding plate driving mechanism. The guiding plate is located in the banknote accommodating space and pivoted with the frame, and the guiding plate driving mechanism drives the guiding plate to rotate from a guiding position to a lifting position. The guiding plate shields the pickuping roller when the guiding plate is at the guiding position, and the guiding plate exposes the pickuping roller when the guiding plate is at the lifting position.