Embodiments of the present application provide a reference signal transmission method, a device, and a system. The method includes: determining, by a first device, at least one reference signal generation sequence corresponding to at least one frequency-domain resource group that is on a symbol carrying a reference signal and that is used to send the reference signal, where one frequency-domain resource group is corresponding to one reference signal generation sequence; and generating, by the first device, the reference signal based on the at least one reference signal generation sequence, and mapping the reference signal to a time-frequency resource whose time domain is the symbol and whose frequency domain is the at least one frequency-domain resource group.