Provided are a display substrate and a preparation method thereof, and a display apparatus. The display substrate includes a plurality of display units. The display unit includes a display area and a transparent area. The display area includes a plurality of sub-pixels. In a direction perpendicular to the display substrate, the sub-pixel includes a first metal layer, a semiconductor layer, a second metal layer and a third metal layer which are arranged on a substrate. The first metal layer includes a first plate, the semiconductor layer includes a second plate, the second metal layer includes a first scanning line and a second scanning line, and the third metal layer includes a third plate, a first power supply line, a second power supply line, a compensation line and a data line.