A disinfecting system and method with monitoring and safety system. The system is available at a semiconductor level with sensors for enabling effective disinfection and human machine interface functions. UV transmissive material can assist in effective distribution of UV-C light for disinfection and visible light for feedback. A replaceable sealed UV source can disinfect multiple distinct areas and be configured with a wide variety of equipment, such as medical equipment, vacuums, and cabinetry. Disinfection interlocks promote safety and efficiency.