A machine tool comprises a tool carrier, e.g. in the form of a turret disc (7) that is provided with receptacles (8) for tool holders (9), wherein each tool holder and tool carrier comprise support surfaces associated to each other and devices for fixing the tool holder at the tool carrier. At least one clamping element is provided at the tool carrier (7) that is adjustably supported between a clamping position and a release position in a direction transverse to a tie rod of a tool holder arranged on a slider (22) receptacle of the tool carrier. The clamping element is provided with a first clamping surface (24) that can be brought into engagement with a second clamping surface (35) at the tie rod of the tool holder in a self-locking and releasable manner.