The present invention is a pressure coupling chamber (1) developed for comparison calibrations of acoustic pressure-sensitive hydrophone (2) in the frequency range from Hz to 1 kHz. A hydrophone (2), reference receiver unit (3) and acoustic source (6) under test are acoustically coupled in the chamber through the air-filled medium inside the pressure coupling chamber (1). Acoustic sources (6) that are going to create acoustic pressure in the pressure coupling chamber (1), placed on the side walls of the chamber (1) to surround the pressure-sensitive active surface of hydrophone (2), are driven to create a hydrostatic pressure effect in the related frequency range in the chamber. Calibration of tested hydrophone (2) with comparison method is carried out by measuring the output voltages of tested hydrophone (2) and the reference receiver unit (3).