A perfusive tissue phantom is disclosed. An example tissue phantom includes a chamber with fluid inlets disposed on a first side and fluid outlets disposed on a second side spaced apart from the first side. The tissue phantom also includes a hydrogel region connected to at least one of the fluid inlets and including a permeable hydrogel with structural characteristics that simulate a type of tissue. The tissue phantom also includes a microfluidic conduit coupled to at least one of the fluid inlets. The microfluidic conduit is a flexible, semi-permeable fiber that simulates a blood vessel. The structural characteristics of the permeable hydrogel may include a selected density, porosity, rigidity and dimension that approximate a target density, porosity, rigidity and dimension of interstitial space of the type of tissue.