- 专利标题: Methods for assembling nucleic acids
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申请号: US17185609申请日: 2021-02-25
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公开(公告)号: US12018316B2公开(公告)日: 2024-06-25
- 发明人: John E. Gill , Lixia Fu , Daniel G. Gibson
- 申请人: CODEX DNA, INC.
- 申请人地址: US CA San Diego
- 专利权人: TELESIS BIO INC.
- 当前专利权人: TELESIS BIO INC.
- 当前专利权人地址: US CA San Diego
- 代理商 Richard San Pietro
- 主分类号: C12Q1/6806
- IPC分类号: C12Q1/6806 ; C12P19/34 ; C12Q1/6862
摘要:
The invention provides methods of assembling a DNA molecule having a desired sequence. The methods involve contacting a DNA ligase with a plurality of short oligonucleotides to be assembled and performing the ligase chain reaction to thereby generate a set of polynucleotides. Oligonucleotides in the plurality overlap with and are complementary to a sequence of at least one other oligonucleotide in the plurality, and at least 50% of the oligonucleotides in the plurality are 6-30 nucleotides in length. The set of polynucleotides produced are contacted with a DNA polymerase and dNTPs in a mixture to join the set of polynucleotides and thereby create a DNA molecule having a desired sequence by polymerase chain assembly. The method allows for production of oligonucleotides of any length having very high sequence fidelity to a desired sequence.
公开/授权文献
- US20210277446A1 METHODS FOR ASSEMBLING NUCLEIC ACIDS 公开/授权日:2021-09-09
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