An endodontic file holder includes a wearing member worn on a part of the body; a holder body mounted on the outer side of the wearing member, having an inner side in which an accommodating space for accommodating a sponge is formed, and having openings through which the sponge accommodated in the accommodating space is exposed; a sponge inserted inside of the accommodating space formed in the holder body, wherein an endodontic file is inserted into the sponge; and a length measuring means provided in the holder body and configured to measure the length of an endodontic file, wherein at least one medicine receiving groove for accommodating a medicine for dental treatment to be applied to an endodontic file is formed on the upper surface of the holder body.