A driving method of a display panel, a display panel, and a display device are provided. Pixel units are grouped, so that the pixel units include the same number of sub-pixels. Each of the pixel units performs a display grayscale switching in a corresponding switching frame. A number of the sub-pixels having a first display gray level and a number of the sub-pixels having a second display gray level in each of the pixel units are the same. A the frame time of maintaining the first display grayscale by each of the pixel units and a frame time of maintaining the second display grayscale by each of the pixel units are the same.