An automatic sampling device includes a sampling unit, a traveling unit and a power supply unit. The sampling unit includes a push module, which includes a push mechanism and a turning mechanism. A plurality of collection modules are sequentially disposed and can be constrained between the turning mechanism and the push mechanism. The push mechanism is at least configured to push the plurality of collection modules to abut against the turning mechanism. The turning mechanism is at least configured to turn over a designated collection module to a material receiving position, and to enable the receiving port of the designated collection module facing towards a sample outlet of the sampling module.