A battery-powered analyte sensing system includes a printed battery and an analyte sensor. The printed battery includes an anode composed of a non-toxic biocompatible metal, a first carbon-based current collector in electrical contact with the anode, a three-dimensional hierarchical mesoporous carbon-based cathode, a second carbon-based current collector, and an electrolyte layer disposed between the anode and the cathode, the electrolyte layer configured to activate the printed battery when the electrolyte is released into one or both the anode and the cathode. The analyte sensor includes a sensing material and a reactive chemistry additive in the sensing material.