A robot synchronization sealer discharge system is provided. The robot synchronization sealer discharge system is interlocked with a robot for a sealer work of a vehicle factory and includes: a sealer gun mounted on a robot and moving along an application path of a vehicle body to discharge a sealer and a communication unit for receiving a TCP speed of the sealer gun moving based on robot operation. A synchronization controller assigns a unique ID and a received TCP time to the TCP speed and generates a discharge instruction message recording the sealer discharge amount according to the TCP speed. A sealer controller adjusts a discharge of the sealer gun based on the discharge instruction message and feeds back the sealer time according to a discharge completion to the synchronization controller.