Provided is a positioning reference signal generation method, which includes the following steps: allocating a time-frequency resource to a positioning reference signal, where the time-frequency resource includes at least two consecutive symbols; and according to generation information of the positioning reference signal and the time-frequency resource, taking the at least two consecutive symbols as a combined PRS symbol to generate the positioning reference signal which is consecutive in a time domain based on a preset generation mode. Further provided are a positioning reference signal generation apparatus, a base station and a readable storage medium.