An ultra-high molecular weight polyethylene fiber with ultra-high cut resistance includes an ultra-high molecular weight polyethylene matrix and carbon fiber powder particles dispersed therein. The content of the carbon fiber powder particles is 0.25-10 wt %. A method for preparing the ultra-high molecular weight polyethylene fiber with the ultra-high cut resistance and a cut-resistant glove woven therefrom are further provided. The test proves that the glove woven from the ultra-high molecular weight polyethylene fiber with the ultra-high cut resistance is soft and comfortable, and does not have prickling sensation. According to the test of the Standard EN388-2003, the level of the cut-resistant grade ranges from 4 to 5.