Satellites in a non-terrestrial network may provide positioning reference signals (PRS) to user equipment (UE), with which the UE may determine its position using propagation delay difference measurements, such as Time Difference of Arrival (TDOA) measurement. Due to the large distances between satellites and the UE, the propagation delay differences in the PRS received from satellites may exceed half a radio frame, resulting in a frame level timing ambiguity in the differential measurements. The satellites transmit secondary PRS, along with primary PRS, that include timing information to resolve frame level timing ambiguity of the primary PRS. The positioning occasions in the secondary PRS, for example, may be aligned with corresponding positioning occasions primary PRS within each radio frame, and are transmitted with a periodicity that is an integer multiple (greater than 1) of that of the primary PRS to resolve the frame level timing ambiguity of the primary PRS.