A display substrate and a display device are provided. The display substrate includes a base substrate, a plurality of pixels, a plurality of gate lines and a plurality of data lines, wherein the base substrate has a plurality of transparent regions and a plurality of display regions; the pixels are on the base substrate and within the display regions; each pixel includes a plurality of sub pixels; the sub pixels of each pixel are divided into two rows of sub pixels; the gate lines and the data lines are on the base substrate; the sub pixels of a first pixel are connected with the same gate line; the gate line connected with the sub pixels of the first pixel is between the two rows of sub pixels of the first pixel; and the first pixel is any one of the plurality of pixels.