The present disclosure provides a display compensation method and device, and a display panel. The display compensation method includes: determining a central pixel column, a first pixel column, a second pixel column, a third pixel column, and a fourth column, in a predetermined direction; determining a target pixel area according to grayscale values of pixel units contained in each pixel column; and adjusting the grayscale value of each pixel unit in the target pixel area to be equal to one actual grayscale value of a picture to be displayed.