The present disclosure discloses a drive backplane, a manufacturing method thereof and a display panel. The drive backplane includes: a base substrate; a first conductive layer, located on the base substrate; a first flat layer, located in a region, other than a pattern of the first conductive layer, on the base substrate; a second flat layer, located on a side, facing away from the base substrate, of the first conductive layer and the first flat layer, where the second flat layer includes a plurality of first via holes; and a second conductive layer, located on a side, facing away from the base substrate, of the second flat layer, where a pattern of the second conductive layer is electrically connected with the pattern of the first conductive layer through the first via holes.