Provided is a display substrate, including a first transparent display region, a second transparent display region and a non-transparent display region. The display substrate includes a plurality of first pixels disposed in an array in the first transparent display region and a plurality of second pixels. The first pixel includes a first light-emitting element and a first pixel circuit. The first pixel circuits in a same row are electrically connected to a same first gate line. The first pixel circuits in a same column are electrically connected to a same first data line. The second pixel includes a second light-emitting element disposed in the second transparent display region and a second pixel circuit disposed outside the first transparent display region and the second transparent display region. The second light-emitting element and the second pixel circuit are electrically connected by a transparent wire.